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We simultaneously measured the GFP inducibility and RNAP II level in individual spore bags after 24 h of incubation in PBS with 100‐μg/ml doxycycline (also see "Microscope Data Analysis" section). "RNAP II level" is the mCherry fluorescence per spore bag due to the mCherry protein fused to Rpb3, a subunit of RNA polymerase II.
Copyright and License information: ©2020 The Authors. Published under the terms of the CC BY 4.0 license
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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