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2.4. Gingiva ex vivo tissue culture

KJ Klara Janjić
BS Barbara Schädl
OA Oleh Andrukhov
HA Hermann Agis
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Gingiva tissue culture was performed to represent an ex vivo situation.

Gingiva tissue from extracted teeth was cut into pieces of approximately 1 mm3 diameter to match the diameter of three GC spheroids. Tissue pieces were seeded onto 1 cm2 of a collagen membrane (Geistlich Bio‐Gide®; Geistlich Pharma AG) or bone substitute (Geistlich Bio‐Oss®; Geistlich Pharma AG) covering 1 cm2 of plastic. Gingiva ex vivo tissues were cultured for 24 h on each surface.

Tissue pieces of gingiva from four different donors were used for histological and immunohistochemical analysis (n = 4), and tissue pieces of gingiva from six different donors were used for metabolic activity, mRNA level and protein analysis (n = 6).

Copyright and License information:  ©2020 The Authors. Journal of Tissue Engineering and Regenerative Medicine published by John Wiley & Sons Ltd
This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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