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Immunofluorescence

LL Ling Leng
JM Jie Ma
LL Luye Lv
WW Wenjuan Wang
DG Dunqin Gao
YZ Yunping Zhu
ZW Zhihong Wu
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Cells were fixed in 4% formaldehyde for 20 min and washed in PBS. Thereafter, the samples were treated with 0.25% Triton X-100 for 20 min, blocked in 10% serum for 1 h at 25 °C, and incubated with primary antibodies, such as COL17A1 (Cat #ab184996, Abcam), K14 (Cat #ab181595, Abcam), K10 (Cat #ab9026, Abcam), PLEC (Cat #sc-33,649, Proteintech), ITGB1 (Cat #ab170874, Abcam), ITGA6 (Cat #ab20142, Abcam), DSC2 (Cat #60239-1-Ig, Proteintech), DSG1 (Cat # 24587-1-AP, Proteintech), β-catenin (Cat #8480S, Cell Signaling), P63 (Cat #ab735, Abcam), CLDN1 (Cat #13050-1-AP, Proteintech), and Ki67 (Cat #ab15580, Abcam) overnight at 4 °C. Thereafter, the cells were incubated with secondary antibodies for 1 h at 25 °C, counterstained with DAPI, and the samples were sealed with Fluoro-Gel for Photography. The pictures were taken at × 20 or × 40 magnification and analyzed using Volocity Demo (× 64).

Copyright and License information: The Author(s) ©2020
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