Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
After PDT treatment, the cells in the 6-well plates were incubated again for 12 h. Then, the cells were harvested by trypsinization, washed twice with PBS, resuspended and fixed with 70% ice-cold ethanol at 4 °C for 24 h. The fixed cells were harvested, washed twice with PBS, and incubated with PI solution (50 µg/mL PI and 20 µg/mL RNase A) at 37 °C for 30 min in the dark. Finally, the cells were analyzed by flow cytometry.
Copyright and License information: ©2020 Optical Society of America under the terms of the
© 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
