2.4. Alkaline Hydrolysis of the Cutin with KOH/MeOH

Fifty milligrams of S. aculeatissimum or S. myriacanthum cutin was added to 50 mL of 1.5 mol·L⁻¹ methanolic KOH solution, and the mixture was stirred at room temperature for 24 h. After this time, the reaction was filtered, neutralized, and monomers were extracted with CHCl₃-MeOH. The dried extract was weighed, dissolved in CHCl₃-MeOH and analyzed by DIESI-MS (Bruker Daltonics, Biellerica, MA, USA).