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HaCaT and HaSKpwC7 cells were harvested and lysed in RIPA buffer. Protein concentration was determined by 660 nm Protein Assay (Pierce). Indicated amounts were loaded. After separation on a 12% PAA-SDS gel, the bands were visualized using mouse-anti-actin (clone AC-40, 1:2,000) and rabbit-anti-p53 (Cell signalling, #9282, 1:1,000) followed by anti-mouse-IgG-Cy3 and anti-rabbit-IgG-Cy5 (both Jackson ImmunoResearch, 1:3,000).
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