Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
IC50 Determination in Cytotoxicity Experiments
Ask a
question
Favorite
Exponentially growing KCL22, LAMA-84, and K562 cells from routine cultures were seeded in fresh medium at 3 × 105 cells/mL. Cells were treated with dimethyl-sulfoxide (DMSO; vehicle) or increasing doses of TKi at the beginning of incubation (treatment at time 0). Half maximal inhibitory concentration (IC50) values were determined by counting viable cells following trypan blue staining after 72 h of treatment and processing data with GraphPad Prism software (La Jolla, CA, USA).
Copyright and License information: The Author(s) ©2020
Open AccessThis article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License, which permits any non-commercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc/4.0/.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
