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Extraction and determination of phenylalanine ammonia-lyase PAL activity.

FB Fatima Zahra Briache
ME Mounia Ennami
JM Joseph Mbasani-Mansi
AL Assia Lozzi
AA Abdelhadi Abousalim
WR Walid El Rodeny
MA Moez Amri
ZT Zine El Abidine Triqui
RM Rachid Mentag
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Extraction and assay of PAL were carried out according to Solecka and Kacperska (2003) using fresh root tissues from healthy and infested treated plants. PAL activity was determined by measuring the absorbance at 290 nm and expressed as EU/mg root fresh weight/h (one unit of enzyme activity equals the amount of PAL that produced 1 μM of trans-cinnamic acid in 1 h).

Copyright and License information:  ©2020 The Korean Society of Plant Pathology
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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