CN analysis

DNAs extracted from FFPE were hybridized on the MIP-assay using Oncoscan FFPE according to standard protocols (Thermo Fisher Scientific, Schwerte, Germany). For comparison, previously published CN data on 35 cases of NMZL²⁴ and 42 of PTFL²⁶ were used, as well as data on t(14;18)⁺ and t(14;18)⁻ FL (for details see supplemental Methods).^6,7 Gains and losses and CNN-LOH regions were evaluated and visually inspected by using Nexus Biodiscovery version 9.0 software (Biodiscovery, Hawthorne, CA). Chromothripsis-like patterns were evaluated according to previously described criteria.²⁹ The human reference genome was GRCh37/hg19. The CNAs with a minimum size of 100 kb and CNN-LOHs larger than 5 Mb were considered informative.