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Monocrotaline (MCT) induced-PAH model
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Adult male SD rats purchased from Beijing Weitong Lihua Experimental Animal Limited Liability Company weighting 180 to 220 g were used in this study. Rats were administered with an intraperitoneal injection of MCT (60 mg/kg Sigma-Aldrich) to induce PAH or received vehicle as control. Hemodynamic studies were carried out 28 days after MCT or vehicle injection after which the rats were euthanized for lung tissues collection.
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