Gibco (Thermo Fisher Scientific India Pvt. Ltd., Mumbai, India) supplied all cell culture reagents including DMEM. Merck Limited, Mumbai, India, supplied sarkosyl, imidazole, staurosporine, kanamycin, and isopropyl β-D-1-thiogalactopyranoside (IPTG). BD Biosciences (San Jose, CA, USA) supplied Middlebrook 7H11 agar, Middlebrook 7H9 media, and Middlebrook 7H10 media. ELISA kit, toxicity removal kit, and enzymes were obtained from PeproTech (Rocky Hill, NJ, USA), Norgen (Thorold, ON, Canada), and NEB (Massachusetts, USA), respectively. Plastic wares for cell culture were obtained from Corning (USA). All reagents used were of analytical grade.
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