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Flow cytometric cell cycle analysis
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Cell cycle distribution was evaluated by flow cytometric analysis using PI staining. After treatment, HL-60 and THP-1 cells were collected, washed with PBS, and fixed in 70% ethanol at 4 °C for 30 min. The cells were then washed twice in PBS and resuspended in 250 µL of PBS containing 50 µg/mL PI and 100 µg/mL RNase A. After incubation in dark at 37 °C for 30 min, the fluorescence of cells was measured with a FACSCanto flow cytometer (Becton Dickinson, San Jose, CA, USA). Data analysis and acquisition were performed using FACS Diva software (Becton Dickinson).
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