Writhing test

Male mice (20–25 gm) were used in this experiment. 30 min. after the administration of B. crispa (50 and 100 mg/kg i.p), mice were administered an i.p. injection of 0.7 % v/v acetic acid solution. The mice were placed individually in transparent cages and 5 min were allowed to elapse. The number of acid-induced writhes were counted for 20 min. For the purpose of scoring, a writhe was indicated by stretching of the abdomen and/or simultaneous stretching of at least one hind limb. Control animals were injected normal saline (10 ml/kg, i.p.), diclofenac (10 mg/kg, i.p.) was used as a standard drug.