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Cell viability assays

MR Marie D. Ralff
AJ Aakash Jhaveri
JR Jocelyn E. Ray
LZ Lanlan Zhou
AL Avital Lev
KC Kerry S. Campbell
DD David T. Dicker
ER Eric A. Ross
WE Wafik S. El-Deiry
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Cell viability assays were performed as described [18]. For the combination experiments, after 72 hours the media was removed from the ONC201 treated wells and replaced with fresh media containing a vehicle control or one of three doses of rhTRAIL. 4 hours later, cellular viability was quantitated using the CellTiter-Glo luminescent cell viability assay (Promega) according to the manufacturer’s instructions. GraphPad Prism software was used to generate dose-response curves.

Copyright and License information:  ©2020 Ralff et al.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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