After a week of acclimatization, shRNA-A2bR 769-P and Caki-1 cells as well as shRNA-nonsense cells as NC group(5×106) were injected subcutaneously into the right flank of 6-week-old BABL/c nude mice (n = 5 for each group). Tumor volumes were calculated as width2×length/2. On day 40th, the mice were sacrificed and the tumor tissues were removed. The animal protocol used in this study was approved by the Animal Ethics Committee of Central South University.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.