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Double-stranded small interfering RNA (siRNA) against adenovirus E1A gene was prepared by annealing the following RNAs which were designed according to siDirect (http://sidirect2.rnai.jp/): 5ʹ-AUAAUCUAACACAAACUCCUC-3ʹ and 5ʹ-GGAGUUUGUGUUAGAUUAUGU-3ʹ. The resulting siRNA was introduced into HEK293 cells using X-tremeGENE transfection reagent (Roche Diagnostics).
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