All statistical analysis was achieved with SPSS version 20. The Shapiro–Wilk and Levene's tests were employed to analyze normality of data and homogeneity of variance. Regarding the results of the previously described analysis, student's t-test, Z-test, or U Mann–Whitney analysis was used when comparing two samples in accordance with tests assumptions (P < 0.05); for three or more samples, ANOVA with Tukey as post hoc or Kruskal–Wallis tests were used (P < 0.05).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.