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2.4. Column Chromatography on Silica Gel

TC Tafadzwa Chipenzi
GB Genuine Baloyi
TM Tatenda Mudondo
SS Simbarashe Sithole
GC Godloves Fru Chi
SM Stanley Mukanganyama
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The leaf extract was run on a column with 100% hexane initially as the eluting solvent. Batch gradient system column elution was employed starting with a solvent of low polarity to that with high polarity. The batch gradient system was a 20-step gradient elution with a gradual increase of polarity with 100% ethyl acetate. Methanol was then added to 100% EA up to 90% EA and 10% methanol. Fractions of 250 ml were collected and concentrated using a Buchi RII rotary evaporator (BÜCHI Labortechnik AG, Postfach, Switzerland). Thin-layer chromatography was used for the analysis of the collected fractions. The fractions with similar retardation factor values were pooled. The pooled fractions were left to evaporate to dryness for crystals to form. Single spots observed on the developed chromatograms under UV (354 and 365 nm) after staining with sulphuring acid were deemed pure and were subjected to NMR analyses.

Copyright and License information:  ©2020 Tafadzwa Chipenzi et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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