Galleria mellonella infection assay

P. aeruginosa strains were grown in MH with 0.5% arabinose, and serial dilutions of bacterial cell suspensions in saline were injected into G. mellonella larvae as described [⁵³]. Ten larvae were infected with each infecting dose in at least three independent experiments, and infected larvae were incubated at 30°C for up to 4 days to monitor mortality. Survival curves, LD₉₀ and R² values were determined using GraphPad Prism as previously described [⁵⁴].