Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Detection of the cellular adenosine triphosphate in mitochondria

JC Jinxia Chang
QW Qiang Wang
AB Anup Bhetuwal
WL Wenhu Liu
ask Ask a question
Favorite

ATP levels in mitochondria were detected using an ATP Assay Kit (Beyotime, #S0027, China) according to the manufactures' instructions. 100 μL ATP working reagent was added to a 96-well plate and put at room temperature for 5 min and then added with 20 μL of purified mitochondria per-well. The ATP levels were tested using a Multi-mode reader (BioTek) and calculated according to the standard ATP curve.

Copyright and License information:  ©2020 The author(s)
This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A