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2.12. In vivo distribution assay

CZ Chuanmin Zhang
YZ Yinan Zhao
EZ Enxia Zhang
MJ Meilin Jiang
DZ Defu Zhi
HC Huiying Chen
SC Shaohui Cui
YZ Yuhong Zhen
JC Jingnan Cui
SZ Shubiao Zhang
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Approximately 1 × 107 NCI-H460 cells were inoculated into the right armpit of each BALB/c nude mouse. Once the volumes of tumor reached to about 500 mm3, mice were administrated with PTX/siRNA/LS (FAM-siRNA = 0.55 mg/kg, PTX = 8 mg/kg) via tail vein. After the injection, mice were sacrificed at the predetermined intervals. Tumor as well as major organs (liver, lung, heart, spleen, and kidney) were excised and analyzed by a bioluminescence IVIS imaging system (Xenogen, USA).

Copyright and License information:  ©2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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