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Approximately 1 × 107 NCI-H460 cells were inoculated into the right armpit of each BALB/c nude mouse. Once the volumes of tumor reached to about 500 mm3, mice were administrated with PTX/siRNA/LS (FAM-siRNA = 0.55 mg/kg, PTX = 8 mg/kg) via tail vein. After the injection, mice were sacrificed at the predetermined intervals. Tumor as well as major organs (liver, lung, heart, spleen, and kidney) were excised and analyzed by a bioluminescence IVIS imaging system (Xenogen, USA).
Copyright and License information: ©2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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