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The 3D tumour spheroid invasion assay was performed as previously described (Han et al., 2017). The cells with spheroids were photographed every 24 h using Nikon microscopy and quantitatively analysed by ImageJ (NIH, Bethesda, MD, USA).
Copyright and License information: ©2019 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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