2.11. Molecular Modeling of the Proteins

Three-dimensional structures of the Mlp124357 were produced through homology modeling using the online tool QUARK [41]. AtPDI-11 and PtPDI structure homology-modeling was obtained from sequence alignment against the homology template of Protein disulfide-isomerase A3 (PDB: 6eny) using I-TASSER [42]. The binding efficiency of the effector to AtPDI-11 or PtPDI was determined using four different protein-protein docking servers Cluspro, Grammx, Patchdock, and ZDock [43,44,45,46]. The generated protein-protein complexes were visualized and analyzed through PyMOL [47,48].