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Healthy blood donations were obtained from the UW Carbone Cancer Center Translational Science Biocore Biobank under an approved IRB protocol. Blood was diluted 1:2 with PBS + 2% FBS and added to a 50 ml SepMate tube (Stemcell, Vancouver, BC) containing 15 ml of Lymphoprep (Stemcell, Vancouver, BC). The gradients were centrifuged at 1200g for 10 minutes and the PBMCs were poured into a new 50 ml conical tube. PBMCs were washed twice with PBS + 2% FBS before being stored frozen in RPMI + 40% FBS + 10% DMSO.
Copyright and License information: ©2020 The Author(s) 2020. Published by Oxford University Press.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
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