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Caspase-3/7 activity was assessed by using Caspase-Glo 3/7 assay kit (Promega Corporation) essentially as described previously [19]. In brief, cells were seeded at the density of 4×104 cells per well in 96-well plate and treated with leptin as indicated in the figure legends. Caspase-3/7 activity was assessed by measurement of luminescence generated from the cleavage of luminogenic substrate Ac-DEVD-pNA with a micro-plate reader (Fluostar Optima, BMG Labtech, Germany).
Copyright and License information: ©2016 Korean J Physiol Pharmacol
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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