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We prepared Total RNA from whole seedlings tissue (14DAG) using RNA Prep Pure Plant Kit (Tiangen, China). We used ~2 mg of RNA for the synthesis of cDNA using PrimeScript Reagent Kit with gDNA Eraser (Takara, Japan). We performed signal detection, quantification, and normalization using Quant Studio 6 proprietary Software (Life technologies, USA). For all the quantitative analyses, we performed a minimum of three biological replicates, three technical replicates each.
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