Analysis of medium samples

KS Katharina Schimek
SF Stefan Frentzel
KL Karsta Luettich
DB David Bovard
IR Isabel Rütschle
LB Laura Boden
FR Felix Rambo
HE Hendrik Erfurth
ED Eva-Maria Dehne
AW Annika Winter
UM Uwe Marx
JH Julia Hoeng
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Medium samples were collected during the medium exchange every 24–72 h for albumin and LDH analysis. A commercially available ELISA kit was used to measure albumin concentrations (E80–129; Bethyl Laboratories, Montgomery, TX, USA). Absorption was determined by using the FLUOstar Omega microplate reader (BMG Labtech, Ortenberg, Germany) at 450 nm. LDH release into the medium was analysed by using the Indiko Plus analyser (Thermo Fisher Scientific, Waltham, MA, USA). Positive controls (maximum LDH release values) were generated by addition of 1% (v/v) Triton X-100 (VWR).

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