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To investigate the cucurbitacin E’s (CuE) effects on actin filaments, A549 cells were pre-incubated with CuE at 10 nM for 1 h. Further, cells were subjected to 15% CS at 0.3 Hz in the presence of the inhibitor for indicated time points. The effect of CuE and cyclic stretch on cell reorientation and SF reorganization was further examined through immunocytochemistry analysis.
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