2.5. Flow Cytometric Analysis of BMMC

After overnight milk incubation, BMMC were washed and incubated with anti-mouse CD16/CD32 (Mouse BD Fc Block; BD Biosciences) for 15 min on ice, to block non-specific binding sites. Cells were subsequently stained with FcεRI-PE-Cy7 and CD117-APC antibodies (Thermo Fisher Scientific) for 45 min on ice. Viable cells were distinguished using the dead cell dye YO-PRO^®-1 Iodide according to the manufacturer’s instructions (Thermo Fisher Scientific). Cell viability and expression of FcεRI and CD117 were measured on the FACS Canto II (BD Biosciences) and analyzed with FlowLogic Software (Inivai Technologies, Mentone, Australia). Isotype controls were used and cut-off gates for positivity were determined with fluorescence-minus-one controls.