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Generation of anti-4.1N antibody

HW Hua Wang
MP Marilyn Parra
JC John G. Conboy
CH Christopher D. Hillyer
NM Narla Mohandas
XA Xiuli An
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Polyclonal antibody against 4.1N was raised in rabbit using His-tagged recombinant N-terminal headpiece of 4.1N as antigen. The sequence of the peptide is unique to 4.1N and does not share any homology with 4.1R, 4.1G or 4.1B. The antibody was affinity purified on Sulfolink Coupling Gel (Pierce Biotechnology, Inc.). Antibody specificity was examined by Western blotting using recombinant 4.1R, 4.1G, 4.1N and 4.1B. As demonstrated in our previous published study, the 4.1N antibody was highly specific for 4.1N and did not recognize other members of the protein 4.1 family38.

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