The lentivirus vectors of pLKO-Fmod, pLKO.1-control, pWPXL-Fmod, and pWPXL-control were constructed by Guangzhou RiboBio Co., Ltd. (RiboBio, Guangzhou, China). The injections of lentivirus were based on previous reports [16]. We performed an initial dose on day 1 in chicks’ legs, harvested the leg muscle tissues on day 9, then fixed the muscles in 10% formalin.
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