B16F10 melanoma cells derived from C57BL/6 J mouse were purchased from ATCC (Manassas, VA, USA). Cells were incubated in DMEM supplemented with 10 % FBS, 100 U/mL penicillin, and 100 mg/mL streptomycin at 37 °C under 5 % CO2.
Copyright and License information: The Author(s). ©2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this
article to respond.