Statistical Analysis

The statistical software R (version 3.6.3), IBM SPSS Statistics 25 (version 25.0.0.1), GraphPad Prism 8 software (version 8.3.0), Adobe Illustrator CC 2018 (version 22.0.0) were used for the statistical analysis and generation of figures. The value of FAM111A expression level above the median value (7.5 for CGGA, 2.6 for TCGA) were defined as high-expression group and the value of FAM111A expression level below the median was defined as the low-expression group. The GSEA molecular signatures dataset (MSigDB) hallmark gene sets were used to perform pathway analysis (24). The enrichment status estimates of inflammatory response-associated metagenes were obtained using the GSVA package. Correlograms and circus plots were performed using the “circlize” package, and the R packages “ImmuneSubtypeClassifier” was used to identify six immune subtypes, which describe a categorization of tumor-immune status. Other R packages, “ggplot2”, “plotROC”, and “xgboost” were also applied for visualizing the results of data analysis. All statistical tests were two-sided and p < 0.05 was considered a significant difference.