4.14. Carrageenan-Induced Paw Edema

The procedure was adapted from Paiva et al. and Lin et al. [7,50]. Balb/c female mice (n = 8/group) were weighed, randomly distributed and orally treated with vehicle (10 mL kg⁻¹, negative control), dexamethasone (25 mg/kg, positive control) and BcAEF (3, 10 and 30 mg kg⁻¹). After one hour, inflammation was induced by CG inoculation (3% in PBS, 30 µL/paw) into the sub-plantar region of the right hind footpad. The hind paw volume was evaluated using a plethysmometer at 0 (basal volume), 1, 2, 4, 6, and 24 h after CG inoculation and the edema volume was obtained by the difference between basal and experimental hind paw volumes.