Assessment of skin lesions and clinical dermatitis severity score

NC/Nga mice were anesthetized with isoflurane, and pictures of the skin were taken weekly until the end of the experiment. The severity of dermatitis was evaluated once a week by scoring the symptoms visible on the ear, neck, and dorsal skin. The severity of dermatitis was assessed based on the four aspects of erythema/hemorrhage, scarring/dryness, edema, and excoriation/erosion on a scale of 0 (none), 1 (mild), 2 (moderate), and 3 (severe). A total dermatitis score was evaluated as the sum of the scores for each aspect, for a maximum score of 12.³¹