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Preparation of Cell Lysates and Western Blot Analysis
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At experimental endpoints, cells were harvested in 1X Laemmli buffer. Alternatively, insoluble nuclear fractions were prepared from cells as described (21) and boiled in 1X Laemmli buffer. Lysates in Laemmli buffer were subjected to western blot as described (22) using primary antibodies (AR SP107, abcam; AR-441, Santa Cruz, Actin C4, Santa Cruz; Histone H3 ab32356, abcam) diluted 1:1000 and secondary antibodies diluted 1:10,000.
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