The right hind paw volume of the mice was measured at 5 hours after injection of carrageenan or saline. Seventy mL pure water was added into the measuring cup of the paw volume meter (KW-7C; Nanjing Calvin Biotechnology Co., Ltd, Nanjing, Jiangsu, China, Fig. 1). The mouse ankle joint was marked. The mouse’s hind knee joint was held by hand to make the hind foot straighten. Then it was slowly put into the measuring cup. When the horizontal plane overlapped with the measurement mark on the mouse foot, the measurement of was carried out [15].
The paw volume meter in this study. Paw volume meter: KW-7C, Nanjing Calvin Biotechnology Co., Ltd, Nanjing, Jiangsu, China.
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