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Briefly, cells were transiently co-transfected with 200 ng of ERE-Luc reporter with 100 ng of ER-WT, ER-MT, PELP1, SRC1, SRC2, SRC3 or control vectors using Turbofect transfection reagent (Thermo Scientific, Waltham, MA). After 24 hr, cells were treated with either vehicle or ERX-11 for an additional 24 hr. β-galactosidase reporter (50 ng) plasmid was co-transfected and used for data normalization. Cells were lysed in Passive Lysis Buffer, and luciferase activity was measured using the luciferase assay system (Promega, Madison, WI) in a luminometer.
Copyright and License information: ©2017, Raj et al
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
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