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2.8. Cytokine Measurement

CF Claudio Franceschi
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Plasma levels of IL-6 and IL-10 were measured by high sensitivity enzyme-linked immunosorbent assay (ELISA) (America-IBL), according to the manufacturer’s instructions. The choice of these two cytokines reflects their opposite role in inflammaging setting. In particular, elevated IL-6 pro-inflammatory levels are associated with a reduced capacity to reach the extreme limits of human life, whereas high levels of anti-inflammatory IL-10 are present among centenarians [42].

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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