The right superior lung lobe was used for measurement of total protein content and a cytokine profile. First, lungs were weighed, homogenized and dissolved in Luminex buffer (weight to volume ratio 1:5) and the total protein content was measured using a BCA protein assay according to manufacturer’s protocol (Thermo Scientific, USA). Concentrations of IL4, IL5, IL-10, IL13, IL-17, IL33, IFNγ, Eotaxin (CCL11), TARC (CCL17), and MIP3α (CCL20) were measured using a multiplex Mouse Cytokine/Chemokine Magnetic Bead Panels (MILLIPLEX Map Kit; Merck Millipore, Germany) according to manufacturer’s protocol. Plates were analyzed using a MAGPX1023 4002 with Luminex xMAP technology.
Copyright and License information: The Author(s) ©2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this
article to respond.