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Colony Formation Assay

SB Saikat Bhattacharya
JW Jerry L. Workman
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The cells (n = 4000) were plated in triplicate in 60-mm tissue culture plates and were allowed to grow as a monolayer for 14 days. Cells were incubated in the complete culture medium, with media changes every 2–3 days. After 14 days, the cells were fixed with 4% paraformaldehyde for 1 h. The colonies were stained with 0.5% crystal violet (0.5% in 70% ethanol) for 1 h at room temperature, rinsed and air-dried. Images were captured and the quantification of the number and size of the colonies was done using ImageJ.

Copyright and License information: The Author(s) ©2020
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