The gene variants of OXTR SNP rs53576 were analyzed with ddPCR mutation detection assay according to the manufacturer’s instructions (Bio-Rad, Hercules, California, USA). Probes were designed to discriminate between the alleles A and G using two different fluorescent TaqMan probes (Table (Table1).1). The probe detecting the A allele was marked with the fluorophore HEX and the probe detecting the G allele was marked with the fluorophore FAM (Bio-Rad, Hercules, California, USA).
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