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Plasmid transfection

YC Yihong Chen
QW Qiumei Wu
JL Jing Lin
JW Juanbing Wei
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The short hairpin RNAs (shRNAs) specific to DARS-AS1 (sh-DARS-AS1#1/2) and the negative control (NC)-shRNAs (sh-NC), as well as miR-628-5p mimics/inhibitor and NC mimics/inhibitor, were devised and produced by GenePharma (Shanghai, China). Additionally, pcDNA3.1/DARS-AS1 and pcDNA3.1/JAG1 were gotten by inserting corresponding cDNA sequences into pcDNA3.1 vectors which were from Invitrogen. The transfection of above plasmids into HeLa and CaSki cells was realized with the aid of Lipofectamine 3000 (Invitrogen). After 48-h plasmid transfection, cells were collected and used for subsequent experiments. The target sequences of shRNAs were: sh-NC: 5′-CACCGTCTTTCAAGGATATGT-3′; sh-DARS-AS1#1: 5′-CACCGTCTGTTAGGGACACAT-3′; sh-DARS-AS1#2: 5′-CACCGTTTCTTTTAAGGAATG-3′.

Copyright and License information: The Author(s) ©2020
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