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Caspase-3/9 activity assay

SW Sumei Wang
ZP Zhiwei Peng
WL Wenjuan Li
SL Shunqin Long
SX Shujing Xiao
WW Wanyin Wu
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Caspase-3/9 activities were measured using Caspase-3/9 activity assay kit according to manufacturer’s protocol (Cell Signaling Technology Inc. Beverly, MA, USA), as previously performed [13]. Briefly, NSCLC cells (A549 and PC9) treated with FZKA and Gefitinib were harvested and lysed in lysis buffer (4 °C for 30 min). Equal amounts of formulations (100 μL) were loaded onto a 96-well microplate and incubated at 37 °C for 120 min with reaction buffer and the liberated DEVD-p-NA substrate. Then the Caspase3/9 activity was measured using the microplate reader at a wavelength of 405 nm. The Caspase3/9 activities were expressed as percentage of enzyme activity compared to that in the control group (untreated cells).

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