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Forty-eight one-week-old SPF chicks were divided into 4 groups, 12 in each and housed in 4 isolators. Chicks were immunized with 107EID50 dose each of inactivated rDHN3-mF, LaSota vaccine or sterile saline in 0.2 mL by the neck subcutaneous injection, and blood samples from each group were collected on 7, 14, and 21 days post-immunization for HI test. On 21 days post-immunization, all birds were infected with 105EID50 of the virulent DHN3. Trachea and cloaca swabs were collected on 3, 5, and 7 days post-challenge.
Copyright and License information: ©2020 Wang, Huang, Fung, Luo, Ye, Du, Wen, Liu and Chen.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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