2.16. Cellular NO (Nitric Oxide) Measurement in RAW 264.7 Cells

Griess reagent was utilized to calculate the concentration of nitric oxide (NO) in the medium as an indicator of NO output as our previous report [33]. At a count of 1.8 × 10⁵ cells per well, RAW 264.7 cells were inoculated into a 96-well microplate and incubated at 37 °C for 1 h. At different concentrations of MS15 (2.5–20 µM), the cells were then treated with 1 µg/mL LPS (lipopolysaccharide). NO concentration of the supernatants was assessed with the addition of Griess reagent after 24 h incubation. The absorbance of the mixtures was assessed at a wavelength of 520 nm, employing a fluorescence microplate scanner.