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2.11. Bacterial Load Determination
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The bacterial loads of lungs were determined at euthanasia (28 days after infection). The left lung of each mouse was aseptically removed, weighed, homogenized and serial dilutions were plated onto DifcoTM Middlebrook 7H10 Agar (BD Biosciences, Johannesburg, SA) medium with oleic acid-albumin-dextrose-catalase (OADC) supplementation and 0.5% glycerol. The CFU were determined 21 days following incubation at 37 °C. Data are expressed as log10 CFU.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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