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FoxO3 phosphorylation
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Cells were transfected with either pcDNA-HA-hBVR or sihBVR. After 24 h, cells were treated with 40 ng/ml IGF-1 for 0, 1, or 4 h. Cell lysates were analyzed by Western blot test using antibodies to phospho-FoxO3, followed by anti-FoxO3A. Signals were quantified by densitometry, and the phospho-FoxO signal was normalized using that for FoxO3A.
Copyright and License information: ©2016 FASEB
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