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Cells were centrifuged at 800×g onto glass slides, and coverslips were mounted with aqueous mounting medium (Dako) with DAPI (Sigma-Aldrich). Fluorescent signals were analyzed using a Zeiss LSM 700 laser-scanning confocal microscope (Göttingen, Germany). The number of LC3 puncta per cell was quantified as described elsewhere [26]. To estimate the average number of LC3 puncta per cell in each treatment group, 20 cells were randomly selected, and puncta in each cell were manually counted. Results are expressed as the mean of at least three independent experiments.
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Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
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