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Pancreatic MPO assay and histological analysis
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The pancreatic MPO assay was performed on frozen tissue using colorimetry assay kits (Jiancheng Bioengineering Institute, Nanjing, China), according to the manufacturer’s instructions. Four-micrometer sections were stained with hematoxylin and eosin (H&E) to observe the morphological changes under the light microscope. The degree of development of pancreatic lesions was evaluated according to point Spormann scale, as previously described [20]. Histological analysis was conducted in control group, SAP group, and SAP treated with ulinastatin group (30000U/kg).
Copyright and License information: The Author(s). ©2017
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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